ANALYTICAL AND FUNCTIONAL PROTEOMICS

ANALYTICAL AND FUNCTIONAL PROTEOMICS

  1. Production of recombinant proteins

    • Service 1

      Production of recombinant proteins in E. coli, yeast cells, specifically engineered human HEK293 or Chinese hamster cells CHO cells, starting from appropriate plasmids (viral or not) provided by the customer and containing the specific tag-fused cDNA as ready-to-use reagents for transfection into the desired model cells.

      References

      Dr Ileana Ramazzina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790

      Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

      For the prokaryotic expression system:

      Internal price - 1.250 Euro/1 milligram (500 Euro for each additional milligram)

      External price - 2.150 Euro/1 milligram (750 Euro for each additional milligram)

      For the eukaryotic expression system:

      Internal price - 1.750 Euro/1 milligram (500 Euro for each additional milligram)

      External price - 2.850 Euro/1 milligram (750 Euro for each additional milligram)

      For specific eukaryotic expression systems, please request quotation.

    • Service 2

      Cloning of customer-supplied cDNA in plasmids suitable for the production of tagged and untagged recombinant proteins.

      References

      Dr Silvia Rossi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

      Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

      Dr Federica Rizzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903816

      Internal price - 1.200 Euro/plasmid

      External price - 2.500 Euro/plasmid

  2. Purification of proteins and cellular compartments

    • Service 1

      We can purify specific proteins from customer-provided human or animal tissues.

      References

      Dr Ileana Ramazzina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790

      Dr Valeria Naponelli, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790

      Internal price - 1.000-1.700 Euro/protein

      External price – 1.700-2.300 Euro/protein

    • Service 2

      Purification of proteins from cell lines or primary cultures.

      References

      Dr Ileana Ramazzina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790

      Dr Valeria Naponelli, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790

      Internal price (customer-provided cells) - 700 Euro/protein

      Internal price (COMT-provided cells) - 1.500 Euro/protein

      External price (customer-provided cells) - 900 Euro/protein

      External price (COMT-provided cells) - 2.000 Euro/protein

    • Service 3

      Subcellular fractionation (separation of plasma membranes, mitochondria, nuclei and cytoplasmic organelles) and macromolecules separation by ultracentrifugation ( i.e. by CsCl gradient) starting from cell lines provided by the customer or by COMT.

      References

      Prof. Saverio Bettuzzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903803

      Dr Federica Rizzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903816

      Internal price (macromolecule separation) – 1.250 Euro/centrifugation

      External price – 1.900 Euro/centrifugation

      Internal price (subcellular fractionation) – 750 Euro/separation

      External price – 1.250 Euro/separation

  3. Molecular interactions

    • Service 1

      Interaction analyses based upon solid-phase binding assays, with or without agonists or antagonists, between proteins or proteins and glycosaminoglycans customer-supplied or available at COMT.

      References

      Dr Carlotta Alias, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003

      Please request quotation

    • Service 2

      Pull-down assays and co-immunoprecipitations from customer-supplied wild type and genetically engineered cell lines and tissue samples are offered. These analyses can be carried out with antibodies provided by the customer or antibodies available at COMT and analyzed by SDS-PAGE, native PAGE, Western blotting with chemiluminescent or fluorescent detection and mass spectroscopy.

      References

      Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655

      Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608

      Please request quotation