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Production of recombinant proteins
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Service 1
Production of recombinant proteins in E. coli, yeast cells, specifically engineered human HEK293 or Chinese hamster cells CHO cells, starting from appropriate plasmids (viral or not) provided by the customer and containing the specific tag-fused cDNA as ready-to-use reagents for transfection into the desired model cells.
References
Dr Ileana Ramazzina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790
Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608
For the prokaryotic expression system:
Internal price - 1.250 Euro/1 milligram (500 Euro for each additional milligram)
External price - 2.150 Euro/1 milligram (750 Euro for each additional milligram)
For the eukaryotic expression system:
Internal price - 1.750 Euro/1 milligram (500 Euro for each additional milligram)
External price - 2.850 Euro/1 milligram (750 Euro for each additional milligram)
For specific eukaryotic expression systems, please request quotation.
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Service 2
Cloning of customer-supplied cDNA in plasmids suitable for the production of tagged and untagged recombinant proteins.
References
Dr Silvia Rossi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655
Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655
Dr Federica Rizzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903816
Internal price - 1.200 Euro/plasmid
External price - 2.500 Euro/plasmid
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Purification of proteins and cellular compartments
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Service 1
We can purify specific proteins from customer-provided human or animal tissues.
References
Dr Ileana Ramazzina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790
Dr Valeria Naponelli, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790
Internal price - 1.000-1.700 Euro/protein
External price – 1.700-2.300 Euro/protein
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Service 2
Purification of proteins from cell lines or primary cultures.
References
Dr Ileana Ramazzina, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790
Dr Valeria Naponelli, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903790
Internal price (customer-provided cells) - 700 Euro/protein
Internal price (COMT-provided cells) - 1.500 Euro/protein
External price (customer-provided cells) - 900 Euro/protein
External price (COMT-provided cells) - 2.000 Euro/protein
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Service 3
Subcellular fractionation (separation of plasma membranes, mitochondria, nuclei and cytoplasmic organelles) and macromolecules separation by ultracentrifugation ( i.e. by CsCl gradient) starting from cell lines provided by the customer or by COMT.
References
Prof. Saverio Bettuzzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903803
Dr Federica Rizzi, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-903816
Internal price (macromolecule separation) – 1.250 Euro/centrifugation
External price – 1.900 Euro/centrifugation
Internal price (subcellular fractionation) – 750 Euro/separation
External price – 1.250 Euro/separation
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Molecular interactions
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Service 1
Interaction analyses based upon solid-phase binding assays, with or without agonists or antagonists, between proteins or proteins and glycosaminoglycans customer-supplied or available at COMT.
References
Dr Carlotta Alias, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-906003
Please request quotation
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Service 2
Pull-down assays and co-immunoprecipitations from customer-supplied wild type and genetically engineered cell lines and tissue samples are offered. These analyses can be carried out with antibodies provided by the customer or antibodies available at COMT and analyzed by SDS-PAGE, native PAGE, Western blotting with chemiluminescent or fluorescent detection and mass spectroscopy.
References
Dr Elisa Tamburini, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905655
Dr Serena Galati, e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it., Tel. (+39) 0521-905608
Please request quotation
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ANALYTICAL AND FUNCTIONAL PROTEOMICS
